Hypersil Gold hydrophilic interaction liquid chromatography columns

This company’s new line of Hypersil Gold hydrophilic interaction liquid chromatography (HILIC) columns are used for the separation of highly polar compounds, which are notoriously difficult to analyze using standard reversed-phase chromatography. The columns work by incorporating water in the highly organic mobile phase to form an adsorbed water-rich layer on the polar stationary phase surface into which analyte molecules partition. Retention is governed by dipole-dipole interactions and hydrogen bonding mechanisms. The highly organic mobile phase contains low salt levels, making Hypersil Gold HILIC columns ideal for use with electrospray mass spectroscopy. With the ability to retain highly polar and hydrophilic compounds, this new HILIC range of columns has been developed to aid the analysis of compounds that are traditionally difficult to retain using conventional C18 columns. The columns are available with 1.9-, 3- and 5-micrometer particle sizes.