OptiCell cell-culture system

 The OptiCell cell-culture system provides scientists with a device that reduces the time and labor required for experimental steps, such as imaging, cell separation, antibody production, transfection, cloning, shipping of live cells, and short-term cryopreservation. Based on the concept of cell growth on a respiratory active membrane, OptiCell utilizes gas-permeable membranes that enable efficient exchange of O2 and CO2 for optimal cell growth. Less than 6-mm thick, the OptiCell system is comprised of two cell-culture-treated polystyrene film membranes sealed onto a microtiter plate-sized frame, forming a hermetically sealed, sterile growth chamber. Two self-sealing ports provide a sterile fluid path to the chamber interior for the transfer and exchange of cells and media. The system takes up a fraction of the space and half the media volume of 75-cm2 flasks. Cells grow on clear, non-fluorescent polystyrene membrane surfaces enabling observation of live and fixed cyto-staining in situ with any optical device or microscope.