Multiple Reconstructions of P. Putida for Metabolic Engineering Applications

Pseudomonas putida is an industrially relevant species of bacteria. It has Generally recognized as safe (GRAS) status, is amenable to genetic manipulations, and has a robust metabolism and tolerance to several solvents used in industry. These attributes give it the potential to be a versatile platform for the bio-industry. Since 2003 multiple different strains of P. putida with varying capabilities have had their genomes fully sequenced. Previously detailed models of the strain KT2440 have been created, but little has been done about other strains. By creating a multi-strain reconstruction of more than 20 strains of this species, new pathways and usages can be elucidated. A pan- and core-genome can be identified which will aid in identifying central pathways as well as peripheral pathways that can be manipulated to add new capabilities and improve output.