Building an Orthogonal Genetics for Rapid Evolution and Synthetic Biology
International Conference Biomolecular Engineering ICBE
2015
5th ICBE - International Conference on Biomolecular Engineering
General Submissions
High-Throughput Biological Design
Tuesday, January 13, 2015 - 12:50pm to 1:15pm
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Building an Orthogonal Genetics for Rapid Evolution and Synthetic Biology
Chang C. Liu, Departments of Biomedical Engineering and Chemistry, UC Irvine
Abstract: We recently developed an orthogonal DNA replication (OrthoRep) system in the yeast Saccharomyces cerevisiae.1 OrthoRep exploits an unusual selfish DNA element consisting of a DNA plasmidâ??DNA polymerase pair that stably replicates in the cytoplasm of yeast. The spatial and mechanistic isolation of OrthoRep from genomic replication may have fundamental significance for biomolecular engineering and synthetic biology. For biomolecular engineering, it offers a platform for rapid targeted evolution where genes encoded on the OrthoRep system undergo accelerated evolution without any increase in the genomic mutation rate. For synthetic biology, OrthoRep is a platform that should enable the bottom-up construction of synthetic genetic and replication systems in vivo.
In this presentation, I will present the design and implementation of OrthoRep, discuss its use as a system for rapid targeted gene evolution in vivo, and present new data and directions that explore the broader implications of OrthoRep for bioengineering.
1A Ravikumar, A Arrieta, CC Liu. An Orthogonal DNA Replication System in Yeast. Nature Chemical Biology, 10, 175-177 (2014).