Nucleic Acid Detection with CRISPR-Cas13

pathogen detection, genotyping, and disease monitoring. We combine the RNA-

targeting CRISPR effector Cas13a with isothermal amplification to establish a

CRISPR-based diagnostic (CRISPR-Dx), providing rapid DNA or RNA detection

with attomolar sensitivity and single-base mismatch specificity. We use this

Cas13a-based molecular detection platform, termed SHERLOCK, to detect

specific strains of Zika and Dengue virus, distinguish pathogenic bacteria,

genotype human DNA, and identify cell-free tumor DNA mutations.