Engineering of the Human Skin Bacterium Cutibacterium Acnes for Medical Applications
International Conference on Microbiome Engineering
2023
6th International Conference on Microbiome Engineering
Poster Session
Poster Presentation Session A
Friday, December 8, 2023 - 4:50pm to 6:00pm
Cutibacterium acnes, a bacterium that thrives within the sebaceous follicles, is the most abundant human skin microbe and has demonstrated promising engraftment properties when transplanted from a donor to a recipient individual. Despite being an ideal chassis for the development of living skin biotherapeutics, the engineering of C. acnes has long been hampered by the extremely low transformation efficiency and the lack of molecular biology tools available.
Here we reverted this situation and rendered C. acnes an engineerable chassis for skin disorders. First, we increased the transformability of C. acnes by several orders of magnitude. This paved the way for the generation of an engineering toolbox for C. acnes that includes modular plasmids and cloning schemes, a promoter library, a library of fluorescent reporters, and gene expression control tools such as CRISPRi and inducible transcription factors. The use of these tools enables the construction of synthetic gene circuits to control C. acnes behaviour in a programmable manner, and the development of strains that produce and secrete molecules with therapeutic potential.