Myeloid Progenitor Cells-Suspension Cell (K562 cells)

10 liters of myeloid progenitor suspension cells  were grown in a Wave bioreactor. The cells were sterily collected with the UniFuge single use centrifuge, using the AC module.   The viability of the grown cells measured 95%. The suspension cells were grown for 4 days, doubling each day. The diameter size of the cells are 15 -20 micron. The cells reached a final cell density 4.93 million cells/ml at time of harvest. To harvest the cells, the UniFuge centrifugation parameters were feed rate (LPM) and G force. The cells were layered on a PBS buffer; a manual prefill step was utilized by switching supply lines. The suspension cells were collected aseptically. After Harvest, the cells were frozen for 24 hours, thawed and viabilities measured.