Revivals the Mouse Liver Damage Using Induced Pluripotent Stem Cells (iPSC)

Induced pluripotent stem cells (iPSCs) are a type of pluripotent stem cell and could be generate from reprogramming of adult somatic cells. The goal of present study was to culture of iPS cells on feeder free layer and injection of iPS cells to mouse with liver failure for evaluation of effect of these cells. In this study two groups include liver failure mouse and control (with no harm) were used for experiment. IPS cells were cultured on BD Matrigel matrix and sufficient colonies were obtained. Then, mice were anesthetized and mechanically were injured in their liver and the iPS cells were injected via to the tail vein. The healthy of mice after injection were evaluated by biochemical tests. In this period liver dysfunction include variety of symptoms related to blood sugar problems, digestive problems, and immune disorders were not detected. Since 14 days, they were killed and revival effects of iPS cells on liver tissue were examined via pathological investigation. The results showed that mice with injured liver had a same appearance with livers of controls and physical signs of tissue damage were not observed. These findings indicate that iPS cells can revival the liver tissue and recovery the damages of hepatocytes cells.

Keywords: iPSCs, BD Matrigel matrix, Liver damage