Angiogenic Secretome of Xeno-Free Hasc

Background/aims. Molecules of animal or bacterial origin, which pose a risk of zoonoses and/or immune rejection, are commonly used for extraction, culture and cryopreservation of mesenchymal stem cells (MSCs). To evaluate qualitatively, quantitatively and functionality, the xeno-free hASC neovascularization secretome, is a good way to evaluate the revascularization capacity of these cells, and these results could suggest the effect of the xeno-free production and the cell culture time on their regenerative potential. Methods. The neovascularization secretome of hASC from two samples in fourth and seventh passage, produced under xeno-free and standard conditions were evaluated using an antibody array and in vitro, ex vivo and in vivo, angiogenesis functional assays. Results The xeno-free hASC secrete higher concentrations of PDGF-BB and IFN-γ. The fourth passage xeno-free hASC secrete higher concentration of several proangiogenic factors like CCL-13, MMP-1 and 9, VEGF and TGF- β1. The seventh passage xeno-free hASC secrete higher concentration of CXCL-5, VEGFA and D, THPO and VEGFR3. The seventh passage xeno-free hASC, shows an increased capacity in the in vitro and ex vivo angiogenesis assays, and the fourth passage shows also increase in the ex vivo angiogenesis assay. In the hind limb ischemia assay there are no substantial differences between the standard and the xeno-free produced cells. Conclusions. The xeno-free production changed quantitatively the hASC secretome and causes an increased neovascularization capacity of it. Under standard conditions, the cell culture time induces an increase in the secretion of antiangiogenic factors, which is correlated with a reduced neovascularization capacity.