Viable Harvest  and Washing of Stem Cells Using Unifuge, Contnous Single Use Centrifuge

Summary: 10 liters of myeloid progenitor suspension cells were grown in a Wave bioreactor. The cells were aseptically collected with the UniFuge single use centrifuge. The viability of the grown cells measured 95%. The suspension cells were grown for 4 days, doubling each day. The diameter size of the cells are 15 -20 micron. The cells reached a final cell density 4.93 million cells/ml at time of harvest. To harvest the cells, the UniFuge centrifuge parameters were feed rate (LPM) and G force . The cells were layered on a PBS buffer; a manual prefill step was utilized by switching supply lines. The suspension cells were collected aseptically. After Harvest, the cells were frozen for 24 hours, thawed and viabilities measured.

Conclusion: For the Viable collection myeloid progenitor like suspension cells, the UniFuge Single use centrifuge met all three objectives. 1. Collect 93% of cells at 700 x G. 2. Cells maintained > 90 viability after 24 hour thaw. 3. Prefill bowl with PBS buffer prior to feed.