Production of the Human Milk Oligosaccharide 2'-O-Fucosyllactose Using E. coli As a Bacterial Cell Factory

Glycom is a privately held company founded in 2005 in Copenhagen, Denmark, with the ambitious goal to synthesize and commercialize human milk oligosaccharides (HMOs).

HMOs are the third most abundant macronutrient in human milk (5-10 g/l), which comprises approximately 200 distinct complex oligosaccharides. Only trace amounts of these oligosaccharides are present in bovine milk-based infant formula. The most abundant oligosaccharide in human milk is 2’-O-Fucosyllactose (2’FL).

Glycom has combined leading edge science with fermentation technology, strain development, and analytical and synthetic chemistry towards the sustainable and cost-effective bio-production of HMOs on an industrial scale.

We will present the production of 2’FL from strain construction to large scale fermentation, to downstream processing and to quality control of the final product.