Regulation of the Pentose Phosphate Pathway in Saccharomyces Cerevisiae

Saccharomyces cerevisiae is one of the most preferred industrial hosts due to stress tolerance and the ease of genetic manipulation. Numerous efforts have been conducted to extend its substrate range, and a number of studies reported engineered strains fermenting xylose efficiently. However, several questions remain to be solved, and one of them is how the native regulatory systems of engineered S. cerevisiae respond to xylose and its intermediates. In a prior study, we reported that the deletion of PHO13, encoding alkaline phosphatase, enhanced xylose fermentation significantly. Later, we identified some evidence that Pho13 is involved in transcriptional and metabolic regulations of the pentose phosphate pathway. In this presentation, transcriptomic and metabolomic data will be presented, which led to the discovery of a novel regulatory mechanism of the pentose phosphate pathway.