Engineering Autonomous Recombinase Switches

Synthetic modules are both under control of specific regulations which are the result of rational design and of the cell physiology which is the result of growth conditions. In bacteria growth-phase-dependent replication, transcription and translation machineries affect proteins productions and thus, synthetic module behaviors. We decided to use the impact of the cell physiology on protein concentration to control a synthetic module, the Recombinase Addressable Data (RAD), without the need of any costly inducer. We developed several strategies mainly based on a control at the transcriptional level to modify, with growth phases, the production of proteins (integrase and/or excisionase) involved in the RAD module. This growth-phase-dependence can be used as a new and economic tool for research and industrial production of molecules of interest.