Autonomous DNA Nanosystem for Interrogating Molecular Interactions

We describe the use of DNA to create a self-assembling nanosystem for interrogating molecular interactions. The nanosystem contains a rigid supporting frame, a cylindrical central core and a mobile ring that is coaxial with the core. Motion of the ring is influenced by several control elements whose force generating capability is based on the transition of single-stranded DNA to double-stranded DNA. These forces can be directed to act in opposition to adhesive forces between the ring and the frame. We interrogate base stacking adhesion and demonstrate detection of a soluble nucleic acid target using this nanosystem.