Use of Synthetic Sgrna to Improve CRISPR Editing Efficiency

CRISPR has made genome editing accessible for a wide range of cell-types. However, obtaining consistent editing efficiencies remains a challenge. Synthego has developed novel RNA synthesis technology to produce 100-mer sgRNA for CRISPR at a practical scale and price. We demonstrate, through a collaborative effort with key researchers utilizing CRISPR, that Synthego sgRNA produces consistent and superior genome editing in a variety of cell types including adherent mammalian cell lines, primary T-cells, iPSCs and model organism embryos. This technology will help to enable succesful and consistent genome editing for both basic research models and for sensitive primary human cells to be used for therapeutic applications.