Enhance Methanol Tolerability of M. Extorquens AM1 By Adaptive Evolution
Translational Medicine and Bioengineering Conference
2016
Translational Medicine and Bioengineering Conference
Poster Submissions
Poster Session
Saturday, November 12, 2016 - 5:00pm to 7:00pm
Enhance
methanol tolerability of M. extorquens
AM1 by adaptive evolution
M.
extorquens AM1 is the most extensively studied
methylotroph model strain in methylotrophic biosynthesis. Methylotrophic
biosynthesis using methanol as feedstock is a promising and attractive way in
industrial biotechnology for the large productivity and low price of methanol
produced from coal or bio-waste anaerobic fermentation-based biomethane.
However, methanol toxicity to
M. extorquens limits the substrate concentration in the
fermentation broth, and
for this reason the methanol based production processes is
of low productivity and long fermentation time.
To obtain the strain with higher methanol
tolerability, atmospheric and room temperature plasma (ARTP) was used in this study
to obtain mutant strains library. By screening mutant strains in generations cultured
with medium containing 5% methanol (v/v), strain CLY-2533 with stable methanol
tolerability was obtained. The tolerance of CLY-2533 was improved more than 2
times than that of the wild-type strain AM1. The final OD600 of
CLY-2533 was 1.83 times higher than that of the wild strain AM1 in 5% (v/v)
methanol. The specific growth rate of CLY-2533 was improved more than 0.37
times. Furthermore, the production of mevalonic acid (MEV) and final OD600 of
CLY-2533-mvt were 496.44
¡À 7.32mg/L
and 10.31 ¡À 0.32, respectively, which were 59.7% and
40.5% higher than that of
the wild-type strain AM1-mvt.