(493h) Supramolecular Engineering of Self-Assembling High Affinity Polymers for Binding-Triggered Phase Separation and Antibody Purification
AIChE Annual Meeting
2021
2021 Annual Meeting
Separations Division
Next Generation Biomolecules and Bioprocesses
Wednesday, November 10, 2021 - 2:36pm to 2:54pm
Our efforts to better understand the correlation between ligand design optimization and antibody precipitation yield improvements have led us to examine how polymer-protein coacervation is facilitated by multivalent mAb binding with a rationally chosen ligand possessing a flexible linker. We found that the ability of each protein to bind to two different ligands is essential for crosslinking different supramolecular polymers and forming a network of polymer-protein complexes. More importantly, improvements in ligand design through the addition of a flexible linker promotes mAb capture by supramolecular polymers and induces phase separation of densely packed polymer-protein coacervates, leaving the mAb-deficient polymers in the supernatant. Overall, these results enhance our understanding of supramolecular polymer-protein interactions and highlight the promising potential of peptide-based affinity systems for mAb purification in a salt-free environment.