(529g) Self-Sustaining Hybrid Bioinorganic Chassis for Improved Fermentation to Produce Complete Flavored Food
AIChE Annual Meeting
2024
2024 AIChE Annual Meeting
Food, Pharmaceutical & Bioengineering Division
Biomanufacturing of Food and Bioproducts
Wednesday, October 30, 2024 - 2:40pm to 2:58pm
Here, a sustainable hybrid bioinorganic system is conceptualized and proposed as an efficient route to transform acetate (model C2 compound) into complete food. We apply the mixture design of experiments (mixture DoE) to formulate the synthetic consortium comprising engineered yeast, fungus, and bacteria to generate balanced food-grade biomass with desired flavors. For ALE experiments, shake flask studies are done with the pure strains to improve their performance to grow at 15g.L-1 acetate. The DoE-based synthetic consortium chassis is tested for 48h in a 1.5 to 300 L fermenter followed by downstream processing to recover biomass. During fermentation, the growth media is minimal salt media supplemented with yeast extract. Later, the recovered biomass is hydrolyzed at pH 5.5, temperature 50 °C, and agitation of 1×g. The produced hydrolysate serves as growth media for fermentation to utilize and assimilate acetate via synthetic consortium chassis into biomass. Before fermentation studies, the hydrolysate is diluted with de-ionized (DI) water in a ratio of 1:1, supplemented with 15g.L-1 acetate, adjusted to pH 6, and autoclaved. This is the demo of sustainable hybrid bioinorganic chassis-based fermentation technology to produce complete food from C2 compound.
References
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