(92e) Biosynthesis of the Acid by a New Technology and Bioreactors

The usage of a main acidifier and preservative, along with up to date tendencies to increase it consumptive demand in the world, dictates current concern in developing high-rate biological processes and modern chip technology with bioreactors. In this research we used citric acid as main acidifier to production. The choice of technology and bioreactors for biosynthesis of citric acid, as well as the efficiency of the biotechnological system as a whole ,is indicated by the kinetic relation ship for the cultivation of microorganisms and the citric acid production.

Using an adequate mathematical model for biosynthesis kinetics ,one can solve below problems :

1)current concern such as the optimal control of the process

2)the choice of rational process conditions( substrate, replacement, aeration rate, biosynthesis duration, etc )

3) technology and bioreactor implementation of the process

In order to analyze of a complex biotechnological system, it is advisable to formulate its total effectiveness index as an addition function of partial in indices of the subsystems that make up it. We consider a mathematical function with 3 parameters where X are state variables, U are control variables and Y are output parameters.

The analysis of the biotechnological system for citric acid producing allowed us to identify two basic subsystems :

1- The biosynthesis subsystem, where the initial carbon-containing substrate at the stage of microbiological synthesis is transformed into the acid which is excreted to the culture broth by the producing strains during their metabolism ,and membrane module.

2- The further stage of isolation as crystallization and drying.

The optimization criteria Φ which arises out of the parameters, has been defined as for the optimal calculation of the chip bioreactor in accordance with the special algorithm we have made. It has been taking into account the biosynthesis time ,concentration of biomass, citric acid and substrate, and parameters of membrane.

Finally, the approach which is based on the mathematical models of kinetics and membrane module and the general algorithm for optimization, allowed us to design plants for the biosynthesis of citric acid, to predict the optimal process parameters and to optimize the performance of operating biotechnological plants.