The rapid discovery and development of antibody drugs against novel targets has never been more important. While advances in synthetic selection and antibody repertoire mining have enabled the rapid identification of candidate binders, the expression and evaluation of candidate antibodies still remains a major bottleneck in the discovery pipeline due to labor-intensive steps and process-throughput mismatches. This webinar details an innovative workflow that enables the expression and evaluation of hundreds of antibodies in a single experiment.
The new workflow consists of a cell-free DNA assembly step to generate antibody expression templates, a modified E. coli cell-free protein synthesis (CFPS) system to express antibodies, and the AlphaLISA protein-protein interaction assay to evaluate binding. The workflow is capable of processing ten times the number of candidates in less than one-third of the time when compared to comparable cell-based methods.
Scientists, professors, and researchers in the pharmaceutical sector should attend this webinar to learn more about these innovative tools. You’ll learn:
- The importance of neutralizing antibodies as a therapeutic modality for emerging viral pathogens
- The bottlenecks in current antibody discovery pipelines
- The basics of CFPS and how it can be used to screen and manufacture antibodies
- How process bottlenecks can be alleviated with CFPS combined with an Echo® acoustic liquid handler
Sponsored webinars bring technical information from reputable firms and suppliers to AIChE members. The content reflects the views, opinions, and recommendations of the sponsoring organization. AIChE does not warrant or represent, expressly or by implication, the correctness or accuracy of the content of the information presented. As between (1) the AIChE, the presenter and author(s) of this work, their employers, and their employers' officers and directors, and (2) the user/viewer of this work, the user/viewer accepts any legal liability or responsibility whatsoever for the consequence of its use or misuse. Contact information for attendees of this webinar, including email address, will be shared with the sponsoring firm. You will always have the opportunity to unsubscribe from email from that organization.
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